Saturday, May 23, 2020

FPKM vs read counts of RNA-seq data


"A quick example of the technical aspect: 
assume a 1,000 bp transcript. experiment 1 is 5,000,000 total reads and this transcript received 5 bhits. This calculates out to an FPKM of 1.0. But that FPKM is based on only 5 hits which is entirely unreliable. experiment 2 has 100,000,000 total reads and this transcript has 100 hits. This also calculates out to an FPKM of 1.0 however this FPKM is much more reliable as it's based on 100 hits which is a more stable count level. the variance due to aligner error and count methods might only vary that count value by 5% whereas the count of 5 could vary by 80% or more."
 link of the given information: http://seqanswers.com/forums/showthread.php?t=30269

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