Today's lab meeting

Yesterday, we meet with Dr. Voit from Georgia Tech. It looks like their lab meeting are very beneficial to understand systems biology topics in more details. I hope I would take the advantage of having to many nice people in their group.
The topic yesterday was "Single cell particle tracking". The speaker was James Wade one of Dr. Voit's students.

Today in bio125 course notes linear regression

Students seemed to does not know the meaning of Linear Regression. Why we do it? how we fit the linear line to the data does not make sense at all for most of them. The simple and mathematical notion should be introduced before we teach it.

H2O2_LOH manuscript revising

Keep revising the manuscript of  Loss of Heterozygosity especially research and discussion part.
Review the tables and figures before submitting a preprint.

oxidative stress and aging key terms and parameters

CLS : chronological life span is a measure of amount of time taken for a single yeast mother cell to stay alive.
RLS: replicative life span measures the number of times required for a mother cell to stop undergoing cell division
MR : Mitotic recombination is the exchange of genetic information between homologous chromosomes in somatic cells
ROS: reactive oxygen species that has a free radical.
LOH: loss of heterozygosity can be used to measure genomic integrity of cells.
MA: mitotic asymmetry, the generation of two dissimilar daughter cells following mitotic division.
MEt15locus: LOH is detected in this genetic locus via knock out of one allele using a resistance marker.
Cv: a variable in the H2O2 does response curve that represents the middle concentration at which cell viability decreases by half
Cb: represents the middle concentration black colonies on MLA plates.
Tg: Biological survival curve , it represents the time at which there is a 50% decrease in genomic integrity.(Qin et al. 2008)
Tc: Tc represents the midpoint of chronological life span(CLS) (Qin et al. 2008)
L0: A ratio that measures the frequency of LOH events in daughter cell/mother cells

Today in bio125 class bradford protein assay of protein concentration and serial dilution is applied. The goal of this assay is to find the protein quantity (unknown) in each experiment. There are 6 experiments that have 0.9% NaCl + BstStock + 5*Bradford solutions.

                               1                 2             3               4            5           6

NaCl                       :    16                     8               4                  2            1               0
BstStock quantity :    640                  720           760             780         790          800
5*Bradford            :    160                   80              40               20           10             0

uknown = 50 ml +200B+750 ml

We will use the linear regression once we measure the amount of amino acids in each tube. Later to predict an unknown solution's protein amount.

My name is Emine Guven. I study quantitative biology. My interests are cellular aging, VEGF receptors clustering, math modeling of biological systems with a broad focus on data analysis and simulations.This site is reserve as a notebook to keep my studies fresh and open to my students and collaborators. As a young scientist my goal is to become more objective and independent. Thanks for visiting my blog.