Friday, January 22, 2016

Today in bio125 class bradford protein assay of protein concentration and serial dilution is applied. The goal of this assay is to find the protein quantity (unknown) in each experiment. There are 6 experiments that have 0.9% NaCl + BstStock + 5*Bradford solutions.

                               1                 2             3               4            5           6

NaCl                       :    16                     8               4                  2            1               0
BstStock quantity :    640                  720           760             780         790          800
5*Bradford            :    160                   80              40               20           10             0

uknown = 50 ml +200B+750 ml

We will use the linear regression once we measure the amount of amino acids in each tube. Later to predict an unknown solution's protein amount.


No comments:

Post a Comment