Tuesday, January 26, 2016

oxidative stress and aging key terms and parameters

CLS : chronological life span is a measure of amount of time taken for a single yeast mother cell to stay alive.
RLS: replicative life span measures the number of times required for a mother cell to stop undergoing cell division
MR : Mitotic recombination is the exchange of genetic information between homologous chromosomes in somatic cells
ROS: reactive oxygen species that has a free radical.
LOH: loss of heterozygosity can be used to measure genomic integrity of cells.
MA: mitotic asymmetry, the generation of two dissimilar daughter cells following mitotic division.
MEt15locus: LOH is detected in this genetic locus via knock out of one allele using a resistance marker.
Cv: a variable in the H2O2 does response curve that represents the middle concentration at which cell viability decreases by half
Cb: represents the middle concentration black colonies on MLA plates.
Tg: Biological survival curve , it represents the time at which there is a 50% decrease in genomic integrity.(Qin et al. 2008)
Tc: Tc represents the midpoint of chronological life span(CLS) (Qin et al. 2008)
L0: A ratio that measures the frequency of LOH events in daughter cell/mother cells

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