RNA-Seq Quantifiying Transcript Levels

Y_f ~ Poisson(theta_f l_f ) where Y_f is the number of reads coming from a transcript (fragment) f.
Q:why multiplication?
A:because the larger the fragment the more reads there are to pick from
Q: why poisson
A: big N, small P_f => Poisson is proposed.

See the papers for more information for how Poisson model is described and more of techniques for RNA-seq.

Next module is going to be about maximum likelihood estimator , the transcript levels of each transcript within a gene.