Tuesday, November 29, 2016

lab meeting 11/29/2016

1)a-DE gene lists for RNAseq project

Q1: there are various time points between control and treatment. Should we use the consensus DEG list?

 IN the BGI report go to /Differential.../DEGList/
(There is an R package NOISeq)
BGI already did the analysis, we need to do a better job @11,12

check NOISeq in the report page 16/23-17/23-...


do a good job on that parts for a manuscript:

11-Pathway Analysis of DEG: functional enrichment phyer, hypergeormetric_distribution
12- PPI Analysis of DEG

It seems "GeneID" in BGI report are from NCBI. Example GeneID: 57573 is a standard ID.



1)b-Pathway analysis plan for DE gene lists
TODO: There are different sources of human gene/protein networks. We should try several for comparisons
TODO: We should try different clustering method, such as hlcust, mcl, etc (refer to Qin's previous paper for clustering analysis).

  2)a-time-lapsed image analysis for yeast replicative lifespan
       softwares:ImageJ, MATLAB, R

https://www.mendeley.com/groups/ gene -pathways/ pathway analysis

gene set analysis is a basic thing, we also need to do that.

data visualization course would be good for animations of .gifs

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