Wednesday, November 23, 2016

tutorial RNA-seq

How to analyze RNA-Seq data? Find differentially expressed genes in your research

https://www.youtube.com/watch?v=xh_wpWj0AzM

What is RNA-seq?
"using next generation sequencing to reveal the presence and quantity of RNA in a biological sample at a given moment in time."(wikipedia)

* differential expression of RNA-seq and pathway analysis is important
here is a nice set of slides:

http://www.mi.fu-berlin.de/wiki/pub/ABI/GenomicsLecture13Materials/rnaseq2.pdf

* nice discussion FPKM vs RPKM

https://www.biostars.org/p/124826/

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